cAMP NOMAD HTR1A Receptor Cell Line

Description U2OS cell line stably expressing HTR1A Receptor and cAMP Biosensor cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD HTR1A Receptor Cell Line contains U2OS cells stably expressing the following constructs: Human Serotonin 1A Receptor (HTR1A) with no tag cAMP Nomad Biosensor (Red Fluorescence) cAMP NOMAD HTR1A Receptor Cell Line allows to assay compounds analyzing the G-Protein signaling pathway via Gi/o protein activation. When an agonist binds to HTR1A, Gi/o protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified in living cells in a dose-response manner by fluorescence emission in a microplate reader..